doxycycline strep 4, 150 mM NaCl, 1 v v Triton X 100 supplemented with protease inhibitors 1 g ml leupeptin and pepstatin, 2 g ml aprotinin, 1 mM phenylmethylsulfonylfluoride and phosphatase inhibitors 1 mM sodium orthovanadate, 10 mM sodium fluoride
